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Aquaculture Studies 2023, Vol 23, Num, 3     (Pages: AQUAST1097)

Preliminary Safety Assessment of Potential Aquaculture Feed Additives Lactiplantibacillus plantarum BCCa32 and BCCa36 Using Zebrafish Larvae as a Model Organism

Abigail Fernandes 1-2 ,Anoop Kumar Yadav 1 ,Kanchanlata Tungare 3 ,Santosh Jathar 1-4 ,Pamela Jha 2-5 ,Renitta Jobby 1-2

1 Amity University, Amity Institute of Biotechnology, Maharashtra - Pune Expressway, Bhatan, Panvel, Maharashtra 410206, India
2 Amity University, Amity Centre of Excellence in Astrobiology, Maharashtra-Pune Expressway, Bhatan, Panvel, Mumbai, Maharashtra 410206, India
3 School of Biotechnology and Bioinformatics, D. Y. Patil Deemed to be University Sector 15/50, CBD, Belapur, Navi Mumbai 400614, India
4 Municipal Laboratory, Municipal Corporation of Greater Mumbai, Dadar, Mumbai 400028, India
5 Sunandan Divatia School of Science, SVKM’S NMIMS University, Mumbai 400056, India
DOI : 10.4194/AQUAST1097 Viewed : 784 - Downloaded : 443 With ever-increasing food demand, aquaculture has witnessed a boom over the past century. As part of the measures to revolutionize the industry, probiotics have emerged as essential feed additives aiding in producing healthy livestock. Before their incorporation into fish feed, potential probiotic strains need to be tested for safety in a relevant model system; hence Danio rerio has been regarded as the model system of interest for testing aquaculture feed additives. In this study, the safety of two Lactiplantibacillus plantarum isolates, namely BCCa32 and BCCa36 was assessed as potential probiotics by using zebrafish larvae. The probiotic isolates did not show any mortality at lower cell densities; however, higher cell densities of BCCa36 increased larval mortality. Of interest, no gut colonization was noted under any condition. The results of this study serve as proof of the principle that zebrafish is a valuable and relevant model system for aquaculture feed and nutrition-related studies. Keywords : Aquaculture nutrition Danio rerio Probiotics Growth promoter