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¹ Tarım ve Orman Bakanlığı Tarımsal Araştırmalar Genel Müdürlüğü, Akdeniz Su Ürünleri Araştırma Üretme ve Eğitim Enstitüsü Müdürlüğü, Ekoloji ve Kaynak Yönetimi, Antalya/Türkiye DOI : 10.4194/AQUAST2087 Viewed : 28 - Downloaded : 29 In this study, Chlorella minutissima, Botryococcus braunii, and Haematococcus pluvialis were selected to culture in Blue-Green 11 (BG-11) media, remineralized sludge water (RSW), and RSW including trace element solution (RSW+Mn) for 28 days. Post-harvest, amino acid and fatty acid profiles were determined. The total amino acid (ΣAA) content was observed in C. minutissima, measuring 68.62±6.35 g/100g, when cultivated in BG-11 culture media. The highest total fatty acid content was found in B. braunii, calculating 95.19±1.38 g/100g, when cultivated in BG-11 culture media. The total content of monounsaturated fatty acids was high compared to polyunsaturated fatty acids (PUFAs) in all groups. This study demonstrates the potential of C. minutissima, B. braunii, and H. pluvialis as a valuable source of essential amino and fatty acids. Among the 171 amino acid comparisons across different culture media, only five (aspartic acid, alanine, cysteine, phenylalanine, and isoleucine in Botryococcus braunii) exhibited statistically significant differences (P<0.05), suggesting that the overall amino acid composition remained relatively stable despite changes in medium formulation. No statistically significant differences were observed among the microalgae species in terms of their fatty acid profiles. Keywords : Chlorella minutissima Botryococcus braunii Haematococcus pluvialis Nutritional quality