Aquaculture Studies
2018, Vol 18, Num, 2 (Pages: 75-78)
The Effects of Different Sperm Activator Medias on the Velocity and Movement Style Brown Trout (Salmo trutta Linnaeus, 1792) Sperm Cells
Mustafa Erkan Ozgur 1
1 İnönü University, Faculty of Fishery, Department of Aquaculture, 44280, Malatya, Turkey
DOI :
10.4194/2618-6381-v18_2_01
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This study was aimed to determine the effects of Na+, K+ and Ca2+ ion-based different
sperm activator medias (SAM) on the velocity and movement style of Brown trout (Salmo
trutta) sperm cells. SAMs were prepared such as SAM1 (9 mM NaCl, 50 mM Glycine and 20
mM Tris), SAM2 (103 mM NaCl, 40 mM KCl, 20 mM HEPES), SAM3 (125 mM NaCl, 30 mM
Tris), SAM4 (60 mM NaHCO3, 50 mM Tris) and SAM5 (125 mM NaCl, 1 mM CaCl2, 30 mM
Glycine, 20 mM Tris), respectively. It was kept at the same pH: 9.0 for all activators.
According to the study data, the statistically significant increases (P<0.05) were found in the
velocities of sperm cells in SAM5 activator compared to other activators. All activators
showed an effect of increasing and decreasing on the values of linearity and amplitude of
lateral displacement of the spermatozoa head in movement style of sperm cells but these
were found to be statistically insignificant (P>0.05). As a result, unlike other activators, the
presence of the Ca2+ (1 mM) ion in the SAM5 activator has resulted in an increase in the
velocities of sperm cells in brown trout sperm cells.
Keywords :
Salmo trutta, Velocity and movement style, Sperm activator media