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Aquaculture Studies 2015, Vol 15, Num, 4     (Pages: 021-027)

Induction of sex-reversed males using 17 α-methlytestosterone in the gold blotch grouper (Epinephelus costae)

Orhan Tufan EROLDOĞAN 1 , Hatice YILMAZ 2 ,Ece EVLİYAOĞLU 3 ,M. GENÇ 4 ,Ercüment GENÇ 5 , Mevlüt AKTAŞ 6

DOI : 10.17693/yunusae.v15i21958.235752 Viewed : 2309 - Downloaded : 1175 In this study, the effects of 17α-methyltestosterone (MT) for induction of sex reversal in protogynous hermafrodit goldblotch grouper (Epinephelus costae) was investigated. Goldblotch grouper were implanted with 5 mg (MT5) and 10 mg (MT10) 17α-methyltestosterone kg-1 month-1 body month weight five times with 15 days intervals. Five fish were implanted for each treatment. After week 8 post-implantation, 2 fish died in MT10 group. These two fish were dissected and MT10 oocytes were degenerated, and sperm channels of these two fish were observed. After last implantation (week 10 post-implantation), only one fish in MT5 treatment induced advanced stages of spermatogenesis. A philometrid nematode, Philometra lateolabracis (Nematoda: Philometridae) was observed in fish in MT5 treatment. It was assumed that this parasitic nematod inhibits the normal development of the gamates in sex reversed females. Keywords : sex reversal,broodstock management,17α-methyltestosterone